Large carnivores are sensitive to human-caused extirpation due to large home ranges, low population densities, and low reproductive rates. Protected areas help maintain populations by acting as sources, but human-caused mortality, habitat displacement, and edge effects occurring at protected area boundaries may reduce that function. The national parks Banff, Yoho, and Kootenay in the Canadian Rocky Mountains are refugia for large carnivores, including wolverines (Gulo gulo (Linnaeus, 1758)). Despite growing conservation concern, empirical baseline population data for wolverines remain scarce throughout their range, including most of Canada. We hypothesized (i) that in these national parks, wolverine density matched values expected for high-quality habitat, and (ii) that edge effects decreased density towards park boundaries. We conducted systematic non-invasive genetic sampling surveys covering >7000 km² (2011 and 2013). Using spatial capture–recapture models, we estimated mean (±SE) female (1.5 ± 0.3 and 1.4 ± 0.3 wolverine/1000 km²), male (1.8 ± 0.4 and 1.5 ± 0.3 wolverine/1000 km²), and combined (3.3 ± 0.5 and 3.0 ± 0.4 wolverine/1000 km²) densities for 2011 and 2013, respectively. These estimates were lower than predictions based on density extrapolation from nearby high-quality habitat, and density decreased towards park boundaries. To benefit the population, we recommend creating buffer zones around parks that protect female habitat and prohibit harvest.