Rural tourism increases income sources for rural residents, reduces rural population outflow, and preserves cultural traditions. This chapter considers development of agrotourism in a rural region like the Omsk region, and agrotourism potential of each Omsk district is assessed. To assess the agrotourism potential, integral and rating indicators were calculated using secondary data and the assessment of 40 experts. The analysis allowed a rating of Omsk's municipal districts. Based on the results, the Southern forest-steppe zone has the highest potential for the development of agrotourism. Several other districts present good conditions to increase the potential of agrotourism development. Some districts are distinct, but conditions for the development of agrotourism exist in all areas of the Omsk region.