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Abstract The relevance of FT-IR spectroscopy in heritage science has experienced a constant grow in the last two decades owing to analytical peculiarities that make it an extremely useful tool to answer the questions posed by the study and conservation of art-historical and archaeological materials. High versatility, sensitivity and molecular specificity are, in fact, all requirements that FT-IR spectroscopy fulfils allowing for the investigation of the chemical properties of heritage materials spanning from the micro- to the macro-scale and offering a variety of approaches to minimize sample manipulation and maximize extracted information. Molecular identification and localisation at high lateral resolution of organic and inorganic components in micro-samples was, over recently, the mostly exploited use of FT-IR in heritage science; however, benefiting from technological progress and advances in optical materials and components achieved in the last decade, it now stands out also for non-invasive surface analysis of artworks by fully portable instrumentation.