Published in
American Society for Microbiology, Journal of Bacteriology, 14(201), 2019
DOI: 10.1128/jb.00089-19
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Expanding the Clostridioides difficile Genetics Toolbox
Journal article published in 2019 by
Aimee Shen 
This paper was not found in any repository, but could be made available legally by the author.
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Abstract
Clostridioides difficile genetics has rapidly advanced in recent years thanks to the development of tools for allelic replacement and transposon mutagenesis. In this Journal of Bacteriology issue, Müh et al. extend the genetics toolbox by developing a CRISPR interference (CRISPRi) strategy for gene silencing in C. difficile (U.