AbstractBuprofezin, a chitin synthesis inhibitor that can be used for the control of hemipteran pests, especially melon aphid, Aphis gossypii. The impact of low lethal concentrations of buprofezin on the biological parameters and expression profile of CHS1 gene were estimated for two successive generations of A. gossypii. The present result shows that the LC₁₅ and LC₃₀ of buprofezin significantly decreased the fecundity and longevity of both generations. Exposure of F₀ individuals to both concentrations delay the developmental period in F₁. Furthermore, the survival rate, intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rate (R₀) were reduced significantly in progeny generation at both concentrations. However, the reduction in gross reproductive rate (GRR) was observed only at LC₃₀. Although, the mean generation time (T) prolonged substantially at LC₃₀. Additionally, expression of the CHS1 gene was significantly increased in F₀ adults. Significant increase in the relative abundance of CHS1 mRNA transcript was also observed at the juvenile and adult stages of F₁ generation following exposure to LC₁₅ and LC₃₀. Therefore, our results show that buprofezin could affect the biological traits by diminishing the chitin contents owing to the inhibition of chitin synthase activity in the succeeding generation of melon aphid.