A data-based method to distinguish climate and land use change impacts on streamflow has been previously developed and needs further evaluation through a large sample study. This study aims to apply the method to a large sample set of 472 catchments in the United States and Australia. The method calculates the water and energy budget of a catchment which can be translated to climate and land use induced changes in streamflow between two periods: a pre-change and post-change period. Several geographical characteristics (e.g., aridity index, average catchment slope, historical land use) were considered for the interpretation of the results. The results show that in general as expected, an increase of the annual discharge is caused by deforestation and a wetter climate, and a decrease of the annual discharge is caused by afforestation and a drier climate. In addition, changes in streamflow of American catchments are likely caused by a wetter climate, while changes in streamflow of Australian catchments are caused by a wetter or drier climate. It can be concluded that the method performs reasonably well and that the results are best explained by the location of the catchment, the aridity index and historical land use.