Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of different level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva.