Published in
American Society for Microbiology, Journal of Clinical Microbiology, 3(49), p. 1140-1142, 2011
DOI: 10.1128/jcm.02328-10
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- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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Comparison of two Mycoplasma genitalium real-time PCR detection methodologies
Journal article published in 2011 by
Jimmy Twin,
Nicole Taylor,
Suzanne M. Garland,
Jennifer Walker,
Jane S. Hocking 
,
Sepehr N. Tabrizi,
Catriona S. Bradshaw,
Christopher K. Fairley,
Jennifer G. McIntosh
,
Sepehr N. Tabrizi,
Catriona S. Bradshaw,
Christopher K. Fairley,
Jennifer G. McIntosh
This paper is made freely available by the publisher.
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Abstract
ABSTRACT Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified ( P = 0.4399).