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MDPI, International Journal of Molecular Sciences, 12(16), p. 7210-7229, 2015

DOI: 10.3390/ijms16047210

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Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

Journal article published in 2015 by Marketa Kominkova, Petr Michalek, Kristyna Cihalova, Roman Guran, Natalia Cernei, Lukas Nejdl, Kristyna Smerkova, Simona Dostalova, Dagmar Chudobova, Zbynek Heger ORCID, Radek Vesely, Jaromir Gumulec ORCID, Jindrich Kynicky, Kledi Xhaxhiu, Ondrej Zitka and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli OPEN ACCESS Int. J. Mol. Sci. 2015, 16 7211 showed significant differences in resistance to antibiotics. According to the determined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 µM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of " more resistant " E. coli exhibited differences in Km between 0.3 and 0.8 µM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.