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Nature Research, Nature Methods, 10(9), p. 977-980, 2012

DOI: 10.1038/nmeth.2141

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Autonomous screening of C. elegans identifies genes implicated in synaptogenesis

Journal article published in 2012 by Matthew M. Crane, Jeffrey N. Stirman ORCID, Chan-Yen Ou, Peri T. Kurshan, James M. Rehg, Kang Shen, Hang Lu ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Morphometric studies in multicellular organisms are mostly performed manually because of the complexity of multidimensional features and lack of appropriate tools for handling these organisms. Here we present an integrated system to autonomously (i.e. without human supervision) identify and sort mutants with altered subcellular traits in real-time. We performed self-directed screens of synapse formation 100× faster and found both novel genes and phenotypic classes previously unidentified in extensive manual screens.