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American Society for Microbiology, Journal of Clinical Microbiology, 9(57), 2019

DOI: 10.1128/jcm.00827-19

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Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo

Journal article published in 2019 by Tony Li, Placide Mbala-Kingebeni, Samia N. Naccache, Julien Thézé ORCID, Jerome Bouquet, Scot Federman, Sneha Somasekar, Guixia Yu, Claudia Sanchez-San Martin, Asmeeta Achari, Bradley S. Schneider, Anne W. Rimoin, Andrew Rambaut, Justus Nsio, Prime Mulembakani and other authors.
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

We applied metagenomic next-generation sequencing (mNGS) to detect Zaire Ebola virus (EBOV) and other potential pathogens from whole-blood samples from 70 patients with suspected Ebola hemorrhagic fever during a 2014 outbreak in Boende, Democratic Republic of the Congo (DRC) and correlated these findings with clinical symptoms. Twenty of 31 patients (64.5%) tested in Kinshasa, DRC, were EBOV positive by quantitative reverse transcriptase PCR (qRT-PCR).