Published in
American Society for Microbiology, Journal of Clinical Microbiology, 9(57), 2019
DOI: 10.1128/jcm.00827-19
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Metagenomic Next-Generation Sequencing of the 2014 Ebola Virus Disease Outbreak in the Democratic Republic of the Congo
,
Jerome Bouquet,
Scot Federman,
Sneha Somasekar,
Guixia Yu,
Claudia Sanchez-San Martin,
Asmeeta Achari,
Bradley S. Schneider,
Anne W. Rimoin,
Andrew Rambaut,
Justus Nsio,
Prime Mulembakani
and other authors.
Full text: Unavailable
Abstract
We applied metagenomic next-generation sequencing (mNGS) to detect Zaire Ebola virus (EBOV) and other potential pathogens from whole-blood samples from 70 patients with suspected Ebola hemorrhagic fever during a 2014 outbreak in Boende, Democratic Republic of the Congo (DRC) and correlated these findings with clinical symptoms. Twenty of 31 patients (64.5%) tested in Kinshasa, DRC, were EBOV positive by quantitative reverse transcriptase PCR (qRT-PCR).