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American Chemical Society, Analytical Chemistry, 24(83), p. 9370-9377, 2011

DOI: 10.1021/ac2019602

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Multifunctional Encoded Self-Assembling Protein Nanofibrils as Platform for High-Throughput and Multiplexed Detection of Biomolecules

Journal article published in 2011 by Ho-Man Chan, Hung-Wing Li ORCID
This paper was not found in any repository; the policy of its publisher is unknown or unclear.
This paper was not found in any repository; the policy of its publisher is unknown or unclear.

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Abstract

A one-dimensional nanofibrillar array formed by the co-assembly of native and biotin-functionalized beta-amyloid (Aβ) peptide was developed for biomolecule sensing. With the presence of biotin moiety, a variety of biomolecular probes can be conjugated onto the nanofibrils, thus converting the protein assembly into a miniature biosensor. In this work, DNA probes were immobilized onto the fibril for the detection of cDNA sequences. The as-developed "DNA-nanoarray" achieved a detection limit at subattomole level (183 fM in 10 μL). This highly sensitive, yet simple, assay requires a trace amount of sample consumption (