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American Society for Microbiology, Applied and Environmental Microbiology, 12(71), p. 8982-8986, 2005

DOI: 10.1128/aem.71.12.8982-8986.2005

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Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility Testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria

Journal article published in 2005 by Ingo Klare, Carola Konstabel, Sibylle Müller-Bertling, Rolf Reissbrodt, Marc Vancanneyt, Jean Swings, Wolfgang Witte, Geert Huys ORCID, Herman Goossens
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus , Pediococcus , Lactococcus , and Bifidobacterium . A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with l -cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.