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National Institute of Environmental Health Sciences (NIEHS), Environmental Health Perspectives, 10(122), p. 1147-1153, 2014

DOI: 10.1289/ehp.1307892

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Identification of DNA Methylation Changes in Newborns Related to Maternal Smoking during Pregnancy

Journal article published in 2014 by Christina A. Markunas, Zongli Xu ORCID, Sophia Harlid, Paul A. Wade ORCID, Rolv Terje Lie, Jack A. Taylor, Allen James Wilcox
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Background: Maternal smoking during pregnancy is associated with significant infant morbidity and mortality, and may influence later disease risk. One mechanism by which smoking (and other environmental factors) might have long-lasting effects is through epigenetic modifications such as DNA methylation. Objectives: We conducted an epigenome-wide association study (EWAS) investigating alterations in DNA methylation in infants exposed in utero to maternal tobacco smoke, using the Norway Facial Clefts Study. Methods: The Illumina HumanMethylation450 BeadChip was used to assess DNA methylation in whole blood from 889 infants shortly after delivery. Of 889 mothers, 287 reported smoking—twice as many smokers as in any previous EWAS of maternal smoking. CpG sites related to maternal smoking during the first trimester were identified using robust linear regression. Results: We identified 185 CpGs with altered methylation in infants of smokers at genome-wide significance (q-value