We present detection of 64 H II regions, three superbubbles and two optical supernova remnant (SNR) candidates in the nearby irregular galaxy NGC 2366. The SNR candidates were detected by applying [S II]/H? ratio criterion to observations made with the 2-m RCC telescope at Rozhen National Astronomical Observatory in Bulgaria. In this paper we report coordinates, diameters, H? and [S II] fluxes for detected objects across the two fields of view in the NGC 2366 galaxy. Using archival XMM-Newton observations we suggest possible X-ray counterparts of two optical SNR candidates. Also, we discard classification of two previous radio SNR candidates in this galaxy since they appear to be background galaxies.