The concentration of suspended solids in water is one of the quality parameters that can be recovered using remote sensing data. This paper investigates the data obtained using a sensor coupled to an unmanned aerial vehicle (UAV) in order to estimate the concentration of suspended solids in a lake in southern Brazil based on the relation of spectral images and limnological data. The water samples underwent laboratory analysis to determine the concentration of total suspended solids (TSS). The images obtained using the UAV were orthorectified and georeferenced so that the values referring to the near, green, and blue infrared channels were collected at each sampling point to relate with the laboratory data. The prediction of the TSS concentration was performed using regression analysis and artificial neural networks. The obtained results were important for two main reasons. First, although regression methods have been used in remote sensing applications, they may not be adequate to capture the linear and/or non-linear relationships of interest. Second, results show that the integration of UAV in the mapping of water bodies together with the application of neural networks in the data analysis is a promising approach to predict TSS as well as their temporal and spatial variations.