The purpose was to determine differences in acute and chronic hydration status in female student-athletes (n = 40) practicing in moderate, dry conditions (17–25 °C, 30–57% humidity) indoors and outdoors. Body weight and urine samples were recorded before and after exercise as well as fluid intake. Sweat rates expressed as median and interquartile range did not differ, but fluid intake was significantly higher during indoor (0.64 [0.50, 0.83] L/h) vs. outdoor conditions (0.51 [0.43, 0.63] L/h), p = 0.001. Fluid intake compensated for indoor sweat rate but not outdoors. When exercising indoors, 49% of the student-athletes reported urine specific gravity (USG) values >1.020, and 24% of the day after morning samples were scored ≥4 on the color chart rating. The percentages increased to 58% and 31%, respectively, when exercising outdoors (p > 0.05). Thus, fluid intake was higher indoors vs. outdoors but sweat rate did not differ among athletes. Yet, chronic hydration status was impaired in more than 50% of the student-athletes with a discrepancy between USG scores and urine color scores identifying underhydration. This suggest that 24-h fluid intake should be taken into account and that hydration protocols may need to be tailored individually based on urine USG values. Practice location (indoors vs. outdoors) may further complicate hydration protocols.