Vinasse, a byproduct result of the process of distillation and fermentation of sugar cane in the ethanol production, stands out for high availability and nutritional alternative source in pest control. The objective of this study was to evaluate the vinasse potential applied to soil, aiming at the control of Pratylenchus brachyurus in soybean crop. The experimental design was a completely randomized factorial (2 × 11), consisting of two forms of application of vinasse (single and divided into two), in eleven concentrations (0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%), with five replications. The soybean plants were inoculated with a suspension of 4,000 eggs/juveniles of P. brachyurus. 60 days after the first application of vinasse, agronomic variables and parasitism were evaluated. The single vinasse application promoted greater growth and root development. Regarding the nematodes parasitism, the application divided was more efficient in the reduction of juveniles in the root and soil, where the concentrations of vinasse enough to reduce the population of 50% (CL50) of the nematodes were 10.22% and 16.64%, respectively. Whereas, for the other variables such as: nematodes per gram of roots (73.97%), eggs in the root (86%) and total nematodes in roots and soil (67.90%), the greatest reduction was observed in the 20% concentration of vinasse. Therefore, vinasse shows potential as a nematicide, as well as excellent organic fertilizer.