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MyJove Corporation, Journal of Visualized Experiments, 78, 2013

DOI: 10.3791/50786-v

MyJove Corporation, Journal of Visualized Experiments, 78, 2013

DOI: 10.3791/50786

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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Journal article published in 2013 by Diederik Wd D. Kuster, David Barefield, Suresh Govindan, Sakthivel Sadayappan ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Biomarkers are becoming increasingly more important in clinical decision-making, as well as basic science. Diagnosing myocardial infarction (MI) is largely driven by detecting cardiac-specific proteins in patients' serum or plasma as an indicator of myocardial injury. Having recently shown that cardiac myosin binding protein-C (cMyBP-C) is detectable in the serum after MI, we have proposed it as a potential biomarker for MI. Biomarkers are typically detected by traditional sandwich enzyme-linked immunosorbent assays. However, this technique requires a large sample volume, has a small dynamic range, and can measure only one protein at a time.