Dissemin is shutting down on January 1st, 2025

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Future Medicine, BioTechniques, 3(44), p. 417-423, 2008

DOI: 10.2144/000112703

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Optimization of RNA extraction from FFPE tissues for expression profiling in the DASL assay

Journal article published in 2008 by Mark Abramovitz, Maja Ordanic-Kodani, Yuefang Wang, Zhenhong Li, Charles Catzavelos, Mark Bouzyk, George W. Sledge, Carlos S. Moreno ORCID, Brian Leyland-Jones
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension, and ligation) assay in which expression of 502 genes are analyzed simultaneously using as little as 100 ng of input RNA.