Published in
American Society for Microbiology, Journal of Clinical Microbiology, 5(57), 2019
DOI: 10.1128/jcm.01794-18
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Development of the TypeSeq Assay for Detection of 51 Human Papillomavirus Genotypes by Next-Generation Sequencing
Journal article published in 2019 by
Sarah Wagner 
,
David Roberson,
Joseph Boland,
Meredith Yeager,
Michael Cullen,
Lisa Mirabello,
S. Terence Dunn,
Joan Walker,
Rosemary Zuna,
Mark Schiffman,
Nicolas Wentzensen
,
David Roberson,
Joseph Boland,
Meredith Yeager,
Michael Cullen,
Lisa Mirabello,
S. Terence Dunn,
Joan Walker,
Rosemary Zuna,
Mark Schiffman,
Nicolas Wentzensen
This paper is made freely available by the publisher.
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Abstract
We have developed a new human papillomavirus (HPV) genotyping assay for detection of 51 HPV genotypes by next-generation sequencing (NGS). The TypeSeq assay consists of 3 PCR steps that equalize viral load and each type’s amplicon copies prior to genotyping by NGS, thereby maximizing multiple-type sensitivity with minimal sequencing reads.