Published in
SAGE Publications, Natural Product Communications, 9(9), p. 1934578X1400900, 2014
DOI: 10.1177/1934578x1400900913
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Unusual 2(1H)-Pyrazinones Isolated from a Culture of a Brazilian Marine-Derived Streptomyces sp
Journal article published in 2014 by
Sérgio S. Thomasi,
Ana C. Granato,
Luis H. Romano,
Liene Dhooghe,
Eduardo S. P. do Nascimento,
Alberto C. Badino,
Maria F. G. F. da Silva,
Antonio G. Ferreira 
,
Tiago Venâncio
,
Tiago Venâncio
This paper is made freely available by the publisher.
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Abstract
Four new secondary metabolites, giovaninones A-D (1–4), were isolated from an ethyl acetate extract of a culture of a marine-derived Streptomyces strain designated SS99BA-2. Chemical analysis was completely conducted in a coupled automated LC-SPE system with the use of a cryogenic NMR probehead and HRMS. The application of this system to identify, purify and elucidate all the structures is described.