American Heart Association, Stroke, Suppl_1(50), 2019
DOI: 10.1161/str.50.suppl_1.tp143
Full text: Unavailable
Introduction: Despite the availability of effective reperfusion therapies, only a few prove beneficial to the patients after stroke, owing to their limited time window. We had earlier demonstrated that adult brain pericytes develop stemness following ischemia, and these ischemia-induced multipotent stem cells (iSCs) can self-propagate and differentiate into mature neural cells, including neurons (Nakagomi et al. Eur J Neurosci. 2009, Stem Cells. 2009, 2015). More recently, we have shown putative iSCs to be present within post-stroke human cerebral tissues (Tatebayashi K et al. Stem Cells Dev 2017). The current study aimed to establish iSCs from cerebellar infarction of two patients and examine their characteristics. Methods: We isolated iSCs from post-stroke human cerebellum, examined their characteristics by Polymerase chain reaction and immunocytochemistry, and compared their traits with those of others by microarray and flow cytometry analyses. Furthermore, we investigated the cerebellar-iSCs (cl-iSCs) for multi-potency and differentiation into functional neurons. Electrophysiological properties were analyzed using multi-electrode arrays. Results: The isolated cl-iSCs exhibited multi-potency and differentiated into electrophysiologically active neurons (Fig. A). Neurogenic potential was confirmed in single-cell suspensions (Fig. B.C). Highly similar gene expression patterns were observed between pericytes and iSCs (Fig. D), and a strong lineage resemblance between cl-iSCs and cerebral ischemia-induced multi-potent stem cells (cr-iSCs) was revealed, although cl-iSCs differentially expressed certain cerebellum-specific genes. Conclusions: Putative iSCs are present in post-stroke cerebellum and possess region-specific traits, suggesting their potential to regenerate functional neurons following an ischemic stroke.