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American Society for Microbiology, Eukaryotic Cell, 1(5), p. 207-211, 2006

DOI: 10.1128/ec.5.1.207-211.2006

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The akuB <sup>KU80</sup> Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

Journal article published in 2006 by Márcia Eliana da Silva Ferreira, Marcia R. V. Z. Kress ORCID, Marcela Savoldi, Maria Helena S. Goldman, Albert Härtl, Thorsten Heinekamp, Axel A. Brakhage, Gustavo H. Goldman
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuBKU80). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuBKU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuB KU80 had no influence on pathogenicity in a low-dose murine infection model. Copyright © 2006, American Society for Microbiology. All Rights Reserved.