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Springer, Journal of Molecular Medicine, 8(91), p. 951-963, 2013

DOI: 10.1007/s00109-013-1015-3

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Proximal tubule specific knockout of the Na+/H+ exchanger NHE3: effects on bicarbonate absorption and ammonium excretion

Journal article published in 2013 by Hong C. Li, Zhaopeng Du, Sharon Barone, Isabelle Rubera ORCID, Alicia A. McDonough, Michel Tauc ORCID, Kamyar Zahedi, Tong Wang, Manoocher Soleimani
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The existing NHE3 knockout mouse has significant intestinal electrolyte absorption defects, making this model unsuitable for the examination of the role of proximal tubule NHE3 in pathophysiologic states in vivo. To overcome this problem, we generated proximal convoluted tubule-specific KO mice (NHE3-PT KO) by generating and crossing NHE3 floxed mice with the sodium-glucose transporter 2 Cre transgenic mice. The NHE3-PT KO mice have >80 % ablation of NHE3 as determined by immunofluorescence microscopy, western blot, and northern analyses, and show mild metabolic acidosis (serum bicarbonate of 21.2 mEq/l in KO vs. 23.7 mEq/l in WT, p