The purpose of the present research was to study the effect of sex and performance on pacing in short (Run1-10 km, Bike-50 km and Run2-5 km) and long distance (Run1-10 km, Bike-150 km and Run2-30 km) in the Powerman World Championship ‘Powerman Zofingen’. All finishers (n = 6671; women, n = 1037; men, n = 5634) competing either in the short or long distance versions of ‘Powerman Zofingen’ from 2003 to 2017 were analyzed for the time spent in each discipline (Run1, Bike and Run2), and in transition (Tran) from Run1 to Bike (Tran1) and from Bike to Run2 (Tran2). Athletes were ranked in quartile (Q) groups (Q1, Q2, Q3 and Q4), with Q1 the fastest and Q4 the slowest. In short distance, in both sexes, a medium discipline/transition × quartile interaction on relative time was observed (p < 0.001, η2p = 0.103 and η2p = 0.119, respectively), where Q1 was relatively the fastest in Tran1, Tran2 and Run2, and the slowest in Bike (p < 0.001). In long distance, in both sexes, a large discipline/transition × quartile interaction on relative time was observed (p < 0.001, η2p = 0.208 and η2p = 0.180, respectively), where Q1 was relatively the fastest in Tran1, Tran2 and Run2, and the slowest in Bike (p < 0.001). In summary, a similar trend of variation of pacing by performance level was observed in both sexes and distances with the fastest duathletes being the fastest in Run2 and both transitions, and the slowest in Bike.