Introduction. To date, growth centiles of children and adolescents have not been created in Ukraine. Therefore, the aim of this study was to construct reference growth charts for height, weight, and body mass index (BMI) of Ukrainian school-aged children and to compare them with World Health Organization references from 2007 for children’s BMI. Material and Methods. Among the representative sample of 13,712 students (aged 7 to 18 years) who were included in this study, 6,582 (48%) were boys and 7,130 (52%) were girls. Assessments of height, body mass, and BMI of participants were performed. Reference charts were developed using LMS models within the ChartMaker lms program. Results. We present first growth references for height, weight, and body mass index for Ukrainian children and adolescents aged 7 to 18 years. The younger Ukrainian pediatric population (7-13 years) was heavier than population reported in the multiethnic WHO references, while the older (13-18 years) had lower body weight comparing to the same references from WHO. Conclusions. The constructed reference growth charts are a benchmark for following secular trends in Ukraine and are also an optimal clinical tool for health care. We recommend national implementation of the Ukrainian reference growth charts for anthropometric measurements.