Dissemin is shutting down on January 1st, 2025

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Nature Research, Nature Cell Biology, 9(8), p. 1035-1037, 2006

DOI: 10.1038/ncb1467

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Essential CDK1 inhibitory role for separase during meiosis I in vertebrate oocytes

Journal article published in 2006 by Ingo H. Gorr, Alexandra Reis, Dominik Boos, Martin Wühr ORCID, Suzanne Madgwick, Keith T. Jones, Olaf Stemmann
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase–Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.