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Taylor and Francis Group, Epigenetics, 4(9), p. 566-578, 2014

DOI: 10.4161/epi.27711

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Role of BRD4 in hematopoietic differentiation of embryonic stem cells

Journal article published in 2014 by Ramon M. Rodriguez, Beatriz Suarez-Alvarez, Ruben Salvanés, Covadonga Huidobro ORCID, Estela G. Toraño, Jose L. Garcia-Perez, Carlos Lopez-Larrea, Agustin F. Fernandez, Clara Bueno, Pablo Menendez ORCID, Mario F. Fraga ORCID
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This paper is available in a repository.

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Abstract

The bromodomain and extra terminal (BET) protein family member BRD4 is a transcriptional regulator, critical for cell cycle progression and cellular viability. Here, we show that BRD4 plays an important role in embryonic stem cell (ESC) regulation. During differentiation of ESCs, BRD4 expression is upregulated and its gene promoter becomes demethylated. Disruption of BRD4 expression in ESCs did not induce spontaneous differentiation but severely diminished hematoendothelial potential. Although BRD4 regulates c-Myc expression, our data show that the role of BRD4 in hematopoietic commitment is not exclusively mediated by c-Myc. Our results indicate that BRD4 is epigenetically regulated during hematopoietic differentiation ESCs in the context of a still unknown signaling pathway.