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American Society of Hematology, Blood Advances, 21(3), p. 3337-3350, 2019

DOI: 10.1182/bloodadvances.2019000689

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Large-scale in-vitro production of red blood cells from human peripheral blood mononuclear cells

Journal article published in 2019 by Steven Heshusius, Esther Heideveld, Patrick Burger, Marijke Thiel-Valkhof, Erica Sellink, Eszter Varga, Elina Ovchynnikova, Anna Visser, Joost H. A. Martens ORCID, Marieke von Lindern ORCID, Emile van den Akker
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Key Points This article provides a defined GMP-grade medium and erythroid culture protocol, resulting in >90% enucleated RBC. This article provides a high-resolution database of RNA expression dynamics at daily intervals during terminal erythroid differentiation.