Plants exhibit complex responses to change in environmental conditions such as radiant heat flux, water quality, airborne pollutants, and soil contents. We seek to utilize natural chemical and electrophysiological response of plants to develop novel plant-based sensor networks. Our present work focuses on plant responses to nuclear radiation—with the goal of monitoring plant responses as benchmarks for detection and dosimetry. In our study, we used plants including Cactus, Arabidopsis, Dwarf mango (pine), Euymus, and Azela. We demonstrated that these plants Chlorophyll-a (F680) to Chlorophyll-b (F735) ratio can be changed according to the radiation dose amount. The recovery processes and speed are different for different plants.