Published in
American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 7(61), 2017
DOI: 10.1128/aac.00400-17
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The Etest Performed Directly on Blood Culture Bottles Is a Reliable Tool for Detection of Fluconazole-Resistant Candida albicans Isolates
,
Laura Judith Marcos-Zambrano,
Ana Gomez,
Ana Gómez,
Carlos Sánchez,
M. Carmen Martinez-Jimenez,
M. Carmen Martínez-Jiménez,
Emilio Bouza ,
Jesús Guinea
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Abstract
ABSTRACT We assessed the ability of the Etest performed directly on positive blood cultures (ET DIR ) to detect fluconazole susceptibility in 6 fluconazole-resistant and 12 fluconazole-susceptible Candida albicans isolates, according to CLSI M27-A3 and EUCAST EDef 7.2 procedures. Categorical agreement between ET DIR and broth microdilution was 100% when the trays were incubated at 25°C and trailing effect was ruled out. ET DIR is a reliable procedure when screening for the presence of fluconazole resistance in C. albicans .