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Optica, Biomedical Optics Express, 12(2), p. 3267, 2011

DOI: 10.1364/boe.2.003267

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Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

Journal article published in 2011 by Metasebya Solomon, Brian R. White ORCID, Ralph E. Nothdruft, Walter Akers ORCID, Gail Sudlow, Adam T. Eggebrecht, Samuel Achilefu, Joseph P. Culver
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.