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International Union of Crystallography, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 12(63), p. 1014-1016, 2007

DOI: 10.1107/s1744309107045757

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Crystallization and preliminary X-ray diffraction studies of the ISC-like [2Fe–2S] ferredoxin (FdxB) fromPseudomonas putidaJCM 20004

Journal article published in 2007 by Toshio Iwasaki, Daijiro Ohmori, Nobutaka Shimizu, Takashi Kumasaka ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

The iron-sulfur (Fe-S) cluster-biosynthesis (ISC) system of the gamma-proteobacterium Pseudomonas putida JCM 20004 contains a constitutively expressed vertebrate-type [2Fe-2S] ferredoxin, FdxB, which lacks the conserved free cysteine residue near the Fe-S cluster site that has been proposed to function in the catalysis of biological Fe-S cluster assembly in other bacterial homologues. Recombinant FdxB was heterologously overproduced in Escherichia coli, purified and crystallized in its oxidized form by the hanging-drop vapour-diffusion and streak-seeding methods using 1.6 M trisodium citrate dihydrate pH 6.5. The thin needle-shaped crystals diffract to 1.90 A resolution and belong to the hexagonal space group P6(1)22, with unit-cell parameters a = 87.58, c = 73.14 A. The asymmetric unit contains one protein molecule.