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National Academy of Sciences, Proceedings of the National Academy of Sciences, 20(93), p. 10679-10684, 1996

DOI: 10.1073/pnas.93.20.10679

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Targeting nucleic acid secondary structures by antisense oligonucleotides designed through in vitro selection.

Journal article published in 1996 by R. K. Mishra, R. Le Tinévez, Le R. Tinevez, J. J. Toulmé ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Using an in vitro selection approach, we have isolated oligonucleotides that can bind to a DNA hairpin structure. Complex formation of these oligonucleotides with the target hairpin involves some type of triple-stranded structure with noncanonical interaction, as indicated by bandshift assays and footprinting studies. The selected oligomers can block restriction endonuclease cleavage of the target hairpin in a sequence-specific manner. We demonstrate that in vitro selection can extend the antisense approach to functional targeting of secondary structure motifs. This could provide a basis for interfering with regulatory processes mediated by a variety of nucleic acid structures.