Abstract The aim of this study was to quantify soil properties, microbial biodiversity and crop yield under two tillage systems used for winter wheat production in monoculture. The study was conducted in the period 2013-2016, on a long-term field experiment on a silt loam at the Krasne Research Station near Rzeszów, Poland. Traditional tillage involved soil inversion whereas reduced tillage was a non-inversion system. The following soil properties: chemical (soil organic carbon, pH, available P, K, Mg), physical (soil bulk density, water content, stability in water), and biological (the diversity of diatoms) were measured on samples collected throughout the growing season and at harvest. Soil organic carbon content, water content and bulk density in the 0-5 and 5-10 cm layers were greater in reduced tillage than in traditional tillage. Under reduced tillage the amount of readily dispersible clay was reduced giving increased soil stability in water. Soil under reduced tillage had greater diversity of diatoms (139 taxa) than that under traditional tillage (102 taxa). Wheat yields were positively correlated with precipitation, soil water content and soil organic carbon, and negatively correlated with readily dispersible clay.