We investigated the antihyperlipidemic activity of an aqueous extract of a herbal preparation from a combination of six Indian medicinal plants. The current study was undertaken to assess the hypolipidemic, hypocholesterolemic and hypotriglyceridemic potential of the polyherbal extract using Triton WR 1339 (Tyloxapol) induced hyperlipidemia. The animals were divided into four groups'normal control, hyperlipidemic control, hyperlipidemic plus polyherbal extract and hyperlipidemic plus Lovastatin. Hyperlipidemia was induced by single intravenous injection of Triton WR 1339. Intragastric administration of polyherbal extract (500mg/kg of body weight) significantly decreased plasma cholesterol, triglyceride, non-HDL-C and phospholipids levels and increased HDL-C levels. Atherogenic index and triglyceride secretion rate were lowered in the polyherbal extract fed animals when compared to hyperlipidemic animals. Polyherbal extract exhibited quite competitive potential when compared with the reference drug Lovastatin affording a possible alternative therapeutic agent in the treatment of hyperlipidemia.