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Royal Society of Chemistry, Chemical Science, 32(9), p. 6692-6702, 2018

DOI: 10.1039/c8sc01444b

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Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis

This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Loop directed mutagenesis leads to a cupredoxin withthe strongest copper–thiolate bond known to date, high reduction potential and imidazole binding properties.