Published in
Royal Society of Chemistry, Chemical Science, 32(9), p. 6692-6702, 2018
DOI: 10.1039/c8sc01444b
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Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis
Journal article published in 2018 by
Andrés Espinoza-Cara 
,
Ulises Zitare ,
Damián Alvarez-Paggi ,
Sebastián Klinke ,
Lisandro H. Otero ,
Daniel H. Murgida ,
Alejandro J. Vila
,
Ulises Zitare ,
Damián Alvarez-Paggi ,
Sebastián Klinke ,
Lisandro H. Otero ,
Daniel H. Murgida ,
Alejandro J. Vila
This paper is made freely available by the publisher.
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Abstract
Loop directed mutagenesis leads to a cupredoxin withthe strongest copper–thiolate bond known to date, high reduction potential and imidazole binding properties.