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Elsevier, Journal of Biological Chemistry, 7(287), p. 4783-4789, 2012

DOI: 10.1074/jbc.m111.318329

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Interaction Surface and Topology of Get3-Get4-Get5 Protein Complex, Involved in Targeting Tail-anchored Proteins to Endoplasmic Reticulum*

Journal article published in 2011 by Yi-Wei Chang ORCID, Tai-Wen Lin, Yi-Chuan Li ORCID, Yu-Shan Huang, Yuh-Ju Sun, Chwan-Deng Hsiao
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Recent work has uncovered the "GET system," which is responsible for endoplasmic reticulum targeting of tail-anchored proteins. Although structural information and the individual roles of most components of this system have been defined, the interactions and interplay between them remain to be elucidated. Here, we investigated the interactions between Get3 and the Get4-Get5 complex from Saccharomyces cerevisiae. We show that Get3 interacts with Get4-Get5 via an interface dominated by electrostatic forces. Using isothermal titration calorimetry and small-angle x-ray scattering, we further demonstrate that the Get3 homodimer interacts with two copies of the Get4-Get5 complex to form an extended conformation in solution.