Significance Largely overlooked, the viruses of protists have started to attract more attention. Several viruses of the family Totiviridae are currently implicated in the increased pathogenicity of parasitic protozoa such as Leishmania to vertebrate hosts. We conducted a broad survey of RNA viruses within trypanosomatids, one of the iconic groups of protists. These revealed several previously unidentified viral taxa including one designated “ Leishbunyaviridae ” and a highly divergent virus termed “Leptomonas pyrrhocoris ostravirus 1.” Our studies provide important information on the origins as well as the diversity and distribution of viruses within a group of protists related to the human parasite Leishmania .