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American Society for Microbiology, Journal of Clinical Microbiology, 1(45), p. 252-254, 2007

DOI: 10.1128/jcm.01489-06

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Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis

Journal article published in 2007 by Leen Rigouts, Oscar Nolasco, Pim de Rijk, Elie Nduwamahoro, Armand Van Deun, Andy Ramsay, Jorge Arevalo ORCID, Françoise Portaels
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT New rpoB gene primers for detecting Rif r in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif r isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.