Objective: To evaluate the pain degree of the patients with chronic non-cancer pain by using the evaluation model constituted by heart rate variability, anxiety and depression scale and quality of life rating scale. This study also aims toevaluate the efficacy after treatment and nursing intervention. Methods: 100 patients with chronic non-cancer pain treated in the hospital from February 2016 to April 2017 were selected to compare their heart rate variability, score of anxiety and depression, score of quality of life and NRS score before and after treatment and nursing intervention. Results: After treatment and nursing intervention, the heart rate variability time domain SDNN significantly increased (P ＜ 0.05). The score of anxiety and depression was lower than that before intervention (P ＜ 0.05) while the scores of various dimensions of quality of life were higher than those before intervention (P ＜ 0.05). The results also showed that NRS score was lower than that before intervention (P ＜ 0.05). Conclusion: These findings suggest that the measurement combination of heart rate variability, anxiety and depression scale and quality of life rating scale can be used as an evaluation model to evaluate the pain degree of the patients with chronic non-cancer pain the efficacy after treatment and nursing intervention, which is worthy of clinic application.