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Oxford University Press, Nucleic Acids Research, 22(21), p. 5286-5286, 1993

DOI: 10.1093/nar/21.22.5286

Oxford University Press, Nucleic Acids Research, 18(21), p. 4159-4165, 1993

DOI: 10.1093/nar/21.18.4159

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L-DNAs as potential antimessenger oligonucleotides: a reassessment.

Journal article published in 1993 by Anna Garbesi, Ml L. Capobianco ORCID, Massimo L. Capobinanco, Francesco P. Colonna, Luisa Tondelli, Federico Arcamone, Giorgio Manzini, Cornelis W. Hilbers, Jan M. E. Aelen, Marcel J. J. Blommers
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Unnatural L-2'-deoxyribonucleosides L-T, L-dC, L-dA and L-dG were prepared from L-arabinose and assembled, by solution or solid phase synthesis, to give L-oligonucleotides (L-DNAs), which contain all four natural bases. The affinity of these modified oligomers for complementary D-ribo- and D-deoxyribo-oligomers was studied with NMR, UV and CD spectroscopies and mobility shift assay on native PAGE. All experimental results indicate that L-DNAs do not, in general, recognize single-stranded, natural DNA and RNA. Hence, contrary to previous suggestions, it is not possible to envisage their use as wide scope antimessenger agents in the selective control of gene expression.