Two scintillating fiber (Sci-Fi) detectors have been operated in the first deuterium plasma campaign of the Large Helical Device in order to investigate the time evolution of the triton burnup through secondary 14 MeV neutron measurement. Two detectors use scintillating fibers of 1 mm diameter embedded in an aluminum matrix with a length of 10 cm connected to the magnetic field resistant photomultiplier. A detector with 91 fibers was developed in the Los Alamos National Laboratory and has been employed on JT-60U. Another detector with 109 fibers has been developed in the National Institute for Fusion Science. The signals are fed into a discriminator of 300 MHz bandwidth with a pulse counter module for online measurement and a digitizer of 1 GHz sampling with 14 bits to acquire pulse shape information for offline data analysis. The triton burnup ratio has been evaluated shot-by-shot by the 14 MeV neutron measurement of Sci-Fi detectors which are calibrated by using the neutron activation system and the total neutron measurement of the neutron flux monitor using 235U fission chambers.