Dissemin is shutting down on January 1st, 2025

Published in

Elsevier, Computational and Structural Biotechnology Journal, 2(1), p. e201207002, 2012

DOI: 10.5936/csbj.201207002

Links

Tools

Export citation

Search in Google Scholar

Limiting factors in single particle cryo electron tomography

Journal article published in 2012 by Mikhail Kudryashev ORCID, Daniel Castaño-Díez, Henning Stahlberg ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

Full text: Download

Green circle
Preprint: archiving allowed
Green circle
Postprint: archiving allowed
Green circle
Published version: archiving allowed
Data provided by SHERPA/RoMEO

Abstract

Modern methods of cryo electron microscopy and tomography allow visualization of protein nanomachines in their native state at the nanometer scale. Image processing methods including sub-volume averaging applied to repeating macromolecular elements within tomograms allow exploring their structures within the native context of the cell, avoiding the need for protein isolation and purification. Today, many different data acquisition protocols and software solutions are available to researchers to determine average structures of macromolecular complexes and potentially to classify structural intermediates. Here, we list the density maps reported in the literature, and analyze each structure for the chosen instrumental settings, sample conditions, main processing steps, and obtained resolution. We present conclusions that identify factors currently limiting the resolution gained by this approach.