Significance Charge order is a modulation of the electron density and is associated with unconventional phenomena, including colossal magnetoresistance and metal–insulator transitions. Determining how the lattice responds provides insights into the nature and symmetry of the ordered state. Scanning transmission electron microscopy can measure lattice displacements with picometer precision, but its use has been limited to room-temperature phases only. Here, we demonstrate high-resolution imaging at cryogenic temperature and map the nature and evolution of charge order in a manganite. We uncover picometer-scale displacive modulations whose periodicity is strongly locked to the lattice and visualize temperature-dependent phase inhomogeneity in the modulations. These results pave the way to understanding the underlying structure of charge-ordered states and other complex phenomena.