Published in
Oxford University Press, Genetics, 4(191), p. 1381-1386, 2012
DOI: 10.1534/genetics.112.141341
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- Oxford University Press | PDF
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- [www.ncbi.nlm.nih.gov] | PDF
- [boris.unibe.ch]
- [europepmc.org] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
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SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing
Journal article published in 2012 by
Heike Lindner,
Michael T. Raissig,
Christian Sailer,
Hiroko Shimosato-Asano,
Rémy Bruggmann 
,
Ueli Grossniklaus
,
Ueli Grossniklaus
This paper is made freely available by the publisher.
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Abstract
Abstract We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.