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Portland Press, Biochemical Journal, 2(236), p. 609-612, 1986

DOI: 10.1042/bj2360609

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Peptide sequencing by using a combination of partial acid hydrolysis and fast-atom-bombardment mass spectrometry.

Journal article published in 1986 by F. De Angelis ORCID, M. Botta, S. Ceccarelli, R. Nicoletti
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

To overcome the limit of the intensity of ions carrying sequence information in structural determinations of peptides by fast-atom-bombardment m.s., we have developed a method that consists in taking spectra of the peptide acid hydrolysates at different hydrolysis times. Peaks correspond to the oligomers arising from the peptide partial hydrolysis. The sequence can then be identified from the structurally overlapping fragments.